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Anti-Phospho-CARM1 (Ser228) Polyclonal Antibody (Synonyms: p-CARM1 (Ser228), p-CARM1 (S228), CARM1 (p-Ser228)|...)
Catalog No. TMAC-00564 Copy Product Info
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Anti-Phospho-CARM1 (Ser228) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CARM1 (Ser228). Anti-Phospho-CARM1 (Ser228) Polyclonal Antibody can be used in IF,WB.
Anti-Phospho-CARM1 (Ser228) Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAC-00564
Synonyms p-CARM1 (Ser228), p-CARM1 (S228), CARM1 (p-Ser228), CARM1 (p-S228)
Anti-Phospho-CARM1 (Ser228) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CARM1 (Ser228). Anti-Phospho-CARM1 (Ser228) Polyclonal Antibody can be used in IF,WB.
Anti-Phospho-CARM1
(Ser228) Polyclonal Antibody
(Ser228) Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $216 | 7-10 days | 7-10 days | |
| 100 μL | $316 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Phospho-CARM1 (Ser228) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CARM1 (Ser228). Anti-Phospho-CARM1 (Ser228) Polyclonal Antibody can be used in IF,WB. |
| Synonyms | p-CARM1 (Ser228), p-CARM1 (S228), CARM1 (p-Ser228), CARM1 (p-S228) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using CARM1 (Ab-228) antibody (Line 1 and 2) and CARM1 (Phospho-Ser228) antibody (Line 3 and 4). 2. Immunofluorescence staining of methanol-fixed HeLa cells using CARM1 (Phospho-Ser228) antibody (TMAC-00564, Red).   |
| Application | |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of serine 228(V-K-S(p)-N-N) derived from Human CARM1 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 63 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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