Anti-Phospho-c-Jun (Thr91+Thr93) Antibody (6U57) is a Rabbit antibody targeting Phospho-c-Jun (Thr91+Thr93). Anti-Phospho-c-Jun (Thr91+Thr93) Antibody (6U57) can be used in WB,IHC-P,IHC-Fr,IP,IF.

IgG

6U57

Human,Mouse

1. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (TMAB-10508) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (TMAB-10508) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (TMAB-10508) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (TMAB-10508) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (TMAB-10508) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (TMAB-10508) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with c-Jun & JunD (phospho-S73/S100) Monoclonal Antibody, Unconjugated (TMAB-10508) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. NIH/3T3 (M) cells were treated with or without anisomycin (25μg/mL) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-c-Jun (Thr91+Thr93) monoclonal antibody, unconjugated (TMAB-10508) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IP: 1:20-50; IF: 1:50-200

Monoclonal

A synthesized peptide: human c Jun around the phosphorylation site of S73

Human

JUN

Transcription factor AP-1

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.