Anti-Phospho-c-Fos (Ser32) Polyclonal Antibody is a Rabbit antibody targeting Phospho-c-Fos (Ser32). Anti-Phospho-c-Fos (Ser32) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Rat (predicted:Mouse)

1. Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-Phospho-c-Fos (Ser32) /AF488 antibody (TMAB-10490-AF488), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-c-Fos (Ser32)) Polyclonal Antibody, Unconjugated (TMAB-10490) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human c-Fos around the phosphorylation site of Ser32

Human

FOS

Proto-oncogene c-Fos

Nuclear phosphoprotein which forms a tight butnon-covalently linked complex with the JUN/AP-1 transcriptionfactor. In the heterodimer, FOS and JUN/AP-1 basic regions eachseems to interact with symmetrical DNA half sites. On TGF-betaactivation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at theAP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Hasa critical function in regulating the Has a critical function inregulating the development of cells destined to form and maintainthe skeleton. It is thought to have an important role in signaltransduction, cell proliferation and differentiation.