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Anti-Phospho-BRAF (Thr401) Antibody (6W728) (Synonyms: p-BRAF (Thr401), p-BRAF (T401), BRAF (p-Thr401), BRAF ...)

Catalog No. TMAC-03193 Copy Product Info
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Anti-Phospho-BRAF (Thr401) Antibody (6W728) is a Rabbit antibody targeting Phospho-BRAF (Thr401). Anti-Phospho-BRAF (Thr401) Antibody (6W728) can be used in ICC,IP,WB.

Anti-Phospho-BRAF (Thr401) Antibody (6W728)

Copy Product Info
🥰Excellent
Catalog No. TMAC-03193
Synonyms p-BRAF (Thr401), p-BRAF (T401), BRAF (p-Thr401), BRAF (p-T401)

Anti-Phospho-BRAF (Thr401) Antibody (6W728) is a Rabbit antibody targeting Phospho-BRAF (Thr401). Anti-Phospho-BRAF (Thr401) Antibody (6W728) can be used in ICC,IP,WB.

Anti-Phospho-BRAF
(Thr401) Antibody
(6W728)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2967-10 days7-10 days
100 μL$4967-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-BRAF (Thr401) Antibody (6W728) is a Rabbit antibody targeting Phospho-BRAF (Thr401). Anti-Phospho-BRAF (Thr401) Antibody (6W728) can be used in ICC,IP,WB.
Synonymsp-BRAF (Thr401), p-BRAF (T401), BRAF (p-Thr401), BRAF (p-T401)
Ig Type
IgG
Clone
6W728
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of Phospho-B Raf (T401) on different lysates using anti-Phospho-B Raf (T401) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse testis, Lane 2: PC-12.
2. ICC staining Phospho-B Raf (T401) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Phospho-B Raf (T401) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Phospho-B Raf (T401) in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:1000-5000; ICC: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundSeveral serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
A synthesized phosphopeptide: human B Raf around the phosphorylation site of Thr401
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 84 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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