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Anti-Phospho-BRAF (Ser365) Polyclonal Antibody 2
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Catalog No. TMAB-10409
Anti-Phospho-BRAF (Ser365) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-BRAF (Ser365). Anti-Phospho-BRAF (Ser365) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-BRAF (Ser365) Polyclonal Antibody 2
Anti-Phospho-BRAF (Ser365) Polyclonal Antibody 2
Copy Product Info😃Good
Catalog No. TMAB-10409
Anti-Phospho-BRAF (Ser365) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-BRAF (Ser365). Anti-Phospho-BRAF (Ser365) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-BRAF (Ser365) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-BRAF (Ser365). Anti-Phospho-BRAF (Ser365) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Rat (predicted:Human,Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (TMAB-10409) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Cell membrane. |
| Tissue Specificity | Brain and testis. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017] |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human BRAF around the phosphorylation site of Ser365 |
| Antigen Species | Human |
| Gene Name | BRAF |
| Gene ID | |
| Protein Name | Serine/threonine-protein kinase B-raf |
| Uniprot ID | |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. |
| Molecular Weight | Theoretical: 84 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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