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Anti-Phospho-BLM (Thr99) Polyclonal Antibody 2
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Catalog No. TMAC-00450
Alias p-BLM (Thr99), p-BLM (T99), BLM (p-Thr99), BLM (p-T99)
Anti-Phospho-BLM (Thr99) Polyclonal Antibody is a Rabbit antibody targeting Phospho-BLM (Thr99). Anti-Phospho-BLM (Thr99) Polyclonal Antibody can be used in WB,IHC.
Anti-Phospho-BLM (Thr99) Polyclonal Antibody 2
Anti-Phospho-BLM (Thr99) Polyclonal Antibody 2
😃Good
Catalog No. TMAC-00450Alias p-BLM (Thr99), p-BLM (T99), BLM (p-Thr99), BLM (p-T99)
Anti-Phospho-BLM (Thr99) Polyclonal Antibody is a Rabbit antibody targeting Phospho-BLM (Thr99). Anti-Phospho-BLM (Thr99) Polyclonal Antibody can be used in WB,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $217 | 7-10 days | |
| 100 μL | $317 | 7-10 days |
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Product Introduction
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Chemical Properties
| Description | Anti-Phospho-BLM (Thr99) Polyclonal Antibody is a Rabbit antibody targeting Phospho-BLM (Thr99). Anti-Phospho-BLM (Thr99) Polyclonal Antibody can be used in WB,IHC. |
| Synonyms | p-BLM (Thr99), p-BLM (T99), BLM (p-Thr99), BLM (p-T99) |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody TMAC-00450. The lane on the right is treated with the antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody TMAC-00450 (left)or the same antibody preincubated with blocking peptide (right). |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE). |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 159 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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