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Anti-Phospho-Bid (Ser61) Polyclonal Antibody
Catalog No. TMAB-10402 Copy Product Info
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Anti-Phospho-Bid (Ser61) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Bid (Ser61). Anti-Phospho-Bid (Ser61) Polyclonal Antibody can be used in FCM.
Anti-Phospho-Bid (Ser61) Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-10402
Anti-Phospho-Bid (Ser61) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Bid (Ser61). Anti-Phospho-Bid (Ser61) Polyclonal Antibody can be used in FCM.
Anti-Phospho-Bid
(Ser61) Polyclonal Antibody
(Ser61) Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-Phospho-Bid (Ser61) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Bid (Ser61). Anti-Phospho-Bid (Ser61) Polyclonal Antibody can be used in FCM. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse) |
| Verified Activity | Blank control: 293T cells (blue). Primary Antibody: Rabbit Anti-PARK7/CAP1 antibody (TMAB-10402), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA. |
| Application | |
| Recommended Dose | FCM: 0.2μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm (By similarity). Mitochondrion membrane. Note=When uncleaved, it is predominantly cytoplasmic. BH3-interacting domain death agonist p15: Mitochondrion membrane. Note=Translocates to mitochondria as an integral membrane protein. BH3-interacting domain death agonist p13: Mitochondrion membrane. Note=Associated with the mitochondrial membran. Isoform 1: Cytoplasm. Isoform 3: Cytoplasm. Isoform 2: Mitochondrion membrane. Note=A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. |
| Tissue Specificity | Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: mouse Bid around the phosphorylation site of Ser61 |
| Antigen Species | Mouse |
| Gene Name | BID |
| Gene ID | |
| Protein Name | BH3-interacting domain death agonist |
| Uniprot ID | |
| Function | The major proteolytic product p15 BID allows the release of cytochrome c. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. |
| Molecular Weight | Theoretical: 21 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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