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Anti-Phospho-BHMT1 (Thr45) Polyclonal Antibody

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Catalog No. TMAB-10401

Anti-Phospho-BHMT1 (Thr45) Polyclonal Antibody is a Rabbit antibody targeting Phospho-BHMT1 (Thr45). Anti-Phospho-BHMT1 (Thr45) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Phospho-BHMT1 (Thr45) Polyclonal Antibody

Anti-Phospho-BHMT1 (Thr45) Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-10401
Anti-Phospho-BHMT1 (Thr45) Polyclonal Antibody is a Rabbit antibody targeting Phospho-BHMT1 (Thr45). Anti-Phospho-BHMT1 (Thr45) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3747-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Phospho-BHMT1 (Thr45) Polyclonal Antibody is a Rabbit antibody targeting Phospho-BHMT1 (Thr45). Anti-Phospho-BHMT1 (Thr45) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1 (Thr45) Polyclonal Antibody, Unconjugated (TMAB-10401) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRPand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1 (Thr45) Polyclonal Antibody, Unconjugated (TMAB-10401) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRPand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1 (Thr45) Polyclonal Antibody, Unconjugated (TMAB-10401) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRPand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1 (Thr45) Polyclonal Antibody, Unconjugated (TMAB-10401) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRPand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Kidney Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-BHMT1 (Thr45) Polyclonal Antibody, Unconjugated (TMAB-10401) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRPand DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
Tissue SpecificityFound exclusively in liver and kidney.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human BHMT1 around the phosphorylation site of Thr45
Antigen Species
Human
Gene Name
BHMT
Gene ID
Protein Name
betaine--homocysteine S-methyltransferase 1
Uniprot ID
Function
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Chemical Properties
Molecular WeightTheoretical: 45 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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