Anti-Phospho-Bcl-xL (Thr47) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-Bcl-xL (Thr47). Anti-Phospho-Bcl-xL (Thr47) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat (predicted:Cow,Horse,Sheep,GuineaPig)

1. Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-BCL2L1) Polyclonal Antibody, Unconjugated (TMAB-10384) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-Bcl-xL (Thr47)) Polyclonal Antibody, Unconjugated (TMAB-10384) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
3. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-Bcl-xL (Thr47)) polyclonal Antibody, Unconjugated (TMAB-10384) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
4. Sample: Lung (Mouse) Lysate at 40 μg
Primary: Anti-p-Bcl-xl (Thr47) (TMAB-10384) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 24 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human BCL2L1 around the phosphorylation site of Thr47

Human

BCL2L1

Bcl-2-like protein 1

Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis.