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Anti-Phospho-Bad (Ser99) Antibody (7Q57)
😃Good
Catalog No. TMAY-01663
Alias BCL2L8, BBC6, BBC2, BAD
Anti-Phospho-Bad (Ser99) Antibody (7Q57) is a Rabbit antibody targeting Phospho-Bad (Ser99). Anti-Phospho-Bad (Ser99) Antibody (7Q57) can be used in WB.
Anti-Phospho-Bad (Ser99) Antibody (7Q57)
Anti-Phospho-Bad (Ser99) Antibody (7Q57)
😃Good
Catalog No. TMAY-01663Alias BCL2L8, BBC6, BBC2, BAD
Anti-Phospho-Bad (Ser99) Antibody (7Q57) is a Rabbit antibody targeting Phospho-Bad (Ser99). Anti-Phospho-Bad (Ser99) Antibody (7Q57) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $80 | 7-10 days | |
| 100 μL | $235 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Phospho-Bad (Ser99) Antibody (7Q57) is a Rabbit antibody targeting Phospho-Bad (Ser99). Anti-Phospho-Bad (Ser99) Antibody (7Q57) can be used in WB. |
| Alias | BCL2L8, BBC6, BBC2, BAD |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 7Q57 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of extracts from serum-starved HEK293 overexpressing BAD, untreated(line A) or treated with forskolin (100nM, 30min; +)(line B), using Phospho-Bad (Ser99) rabbit monoclonal Antibody at 1:1000 dilution. 2. Western blot analysis of extracts from serum-starved HEK293 overexpressing BAD, untreated (line A); treated with forskolin (100nM, 30min; +)(line B); treated with forskolin and λ-phosphatase (line C) using Phospho-Bad (Ser99) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) |
| Application | WB |
| Recommended Dose | WB: 1:1000-1:20000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around Ser99 of Human Phospho-Bad. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | Bcl-2 agonist of cell death (BAD) is a BH3-only member of the Bcl-2 family which possesses important regulatory function in apoptosis. BAD has also been shown to possess many non-apoptotic functions closely linked to cancer including regulation of glycolysis, autophagy, cell cycle progression and immune system development. Oral squamous cell carcinoma (OSCC) is a type of cancer with high morbidity and mortality rates worldwide; it also demonstrates chemotherapeutic resistance. Triterpenoid ursolic acid has been shown to exhibit various biological activities and anticancer effects in several preclinical studies. The apoptosis‑associated signaling showed that ursolic acid decreased the phosphorylation of AKT (Ser473) and B‑cell lymphoma 2 (Bcl‑2)‑associated agonist of cell death (BAD; Ser136), and the protein levels of Bcl‑2 and Bcl‑extra large (Bcl‑xL), and increased the expression of BAD and Bcl‑2‑associated X (Bax) protein in CAR cells. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide corresponding to the residues around Ser99 of Human Phospho-Bad |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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