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Anti-Phospho-arfaptin 2 (Ser260) Polyclonal Antibody
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Catalog No. TMAB-10338
Anti-Phospho-arfaptin 2 (Ser260) Polyclonal Antibody is a Rabbit antibody targeting Phospho-arfaptin 2 (Ser260). Anti-Phospho-arfaptin 2 (Ser260) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-arfaptin 2 (Ser260) Polyclonal Antibody
Anti-Phospho-arfaptin 2 (Ser260) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10338
Anti-Phospho-arfaptin 2 (Ser260) Polyclonal Antibody is a Rabbit antibody targeting Phospho-arfaptin 2 (Ser260). Anti-Phospho-arfaptin 2 (Ser260) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-Phospho-arfaptin 2 (Ser260) Polyclonal Antibody is a Rabbit antibody targeting Phospho-arfaptin 2 (Ser260). Anti-Phospho-arfaptin 2 (Ser260) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Cow,Rabbit,Sheep) |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-arfaptin 2 (Ser260)) Polyclonal Antibody, Unconjugated (TMAB-10338) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | ARFIP2 is a ubiquitously expressed protein implicated in mediating cross talk between RAC and ARF small GTPases. It has been shown that ARFIP2 binds specifically to GTP-bound ARF1 and ARF6, but binds to Rac-GTP and Rac-GDP with similar affinities. The X-ray structure of arfaptin reveals an elongated, crescent-shaped dimer of 3-helix coiled-coils. Structures of arfaptin with Rac bound to either GDP or the slowly hydrolysable analog GMPPNP show that the switch regions adopt similar conformations in both complexes. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human arfaptin 2 around the phosphorylation site of Ser260 |
| Antigen Species | Human |
| Gene Name | ARFIP2 |
| Gene ID | |
| Protein Name | Arfaptin-2 |
| Uniprot ID | |
| Function | Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling. |
| Molecular Weight | Theoretical: 38 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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