Anti-Phospho-APC1 (Ser355) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-APC1 (Ser355). Anti-Phospho-APC1 (Ser355) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Sample: Ovary (Mouse) Lysate at 40 μg
Primary: Anti-phospho-APC1 (Ser355) (TMAB-10324) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 212 kD
Observed band size: 212 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-APC1 (Ser355)) Polyclonal Antibody, Unconjugated (TMAB-10324) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human ANAPC1 around the phosphorylation site of Ser355

Human

ANAPC1

Anaphase-promoting complex subunit 1

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.