Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody 2
😃Good
Catalog No. TMAC-00187
Alias p-AMPKα1/2 (Thr183/172), p-AMPKα1/2 (T183/172), AMPKα1/2 (p-Thr183/172), AMPKα1/2 (p-T183/172)
Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AMPKα1/2 (Thr183/172). Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody can be used in WB,IHC,IF.
Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody 2
Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody 2
😃Good
Catalog No. TMAC-00187Alias p-AMPKα1/2 (Thr183/172), p-AMPKα1/2 (T183/172), AMPKα1/2 (p-Thr183/172), AMPKα1/2 (p-T183/172)
Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AMPKα1/2 (Thr183/172). Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody can be used in WB,IHC,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $216 | 7-10 days | |
| 100 μL | $318 | 7-10 days |
Add to Cart
Add to Quotation
Bulk & Custom
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Questions
View MoreResource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AMPKα1/2 (Thr183/172). Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody can be used in WB,IHC,IF. |
| Synonyms | p-AMPKα1/2 (Thr183/172), p-AMPKα1/2 (T183/172), AMPKα1/2 (p-Thr183/172), AMPKα1/2 (p-T183/172) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western Blot analysis of various cells using Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody diluted at 1:500. 2. Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. 3. Immunofluorescence analysis of Hela cell. 1,AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody(green) was diluted at 1:200(4°C overnight). (red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:100-300; IF: 1:50-200 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Appearance | Liquid |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research Background | Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human AMPK alpha around the phosphorylation site of Thr172 |
| Antigen Species | human |
| Molecular Weight | Actual: 63 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Sci Citations
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Preview
Related Tags: buy Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody 2 | purchase Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody 2 | Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody 2 cost | order Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody 2 | Anti-Phospho-AMPKα1/2 (Thr183/172) Polyclonal Antibody 2 molecular weight
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.