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Anti-Phospho-AMPKα (Ser496) Antibody (3X970) (Synonyms: Phospho-AMPKα (S496), p-AMPKα (Ser496), p-AMPKα (S496)...)

Catalog No. TMAC-03190 Copy Product Info
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Anti-Phospho-AMPKα (Ser496) Antibody (3X970) is a Rabbit antibody targeting Phospho-AMPKα (Ser496). Anti-Phospho-AMPKα (Ser496) Antibody (3X970) can be used in ICC/IF,IP,WB.

Anti-Phospho-AMPKα (Ser496) Antibody (3X970)

Copy Product Info
🥰Excellent
Catalog No. TMAC-03190
Synonyms Phospho-AMPKα (S496), p-AMPKα (Ser496), p-AMPKα (S496), AMPKα (p-Ser496), AMPKα (p-S496), AMPKalpha, AMPKa1, AMPK1, AMPK alpha 1, AMPK

Anti-Phospho-AMPKα (Ser496) Antibody (3X970) is a Rabbit antibody targeting Phospho-AMPKα (Ser496). Anti-Phospho-AMPKα (Ser496) Antibody (3X970) can be used in ICC/IF,IP,WB.

Anti-Phospho-AMPKα
(Ser496) Antibody
(3X970)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2987-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-AMPKα (Ser496) Antibody (3X970) is a Rabbit antibody targeting Phospho-AMPKα (Ser496). Anti-Phospho-AMPKα (Ser496) Antibody (3X970) can be used in ICC/IF,IP,WB.
SynonymsPhospho-AMPKα (S496), p-AMPKα (Ser496), p-AMPKα (S496), AMPKα (p-Ser496), AMPKα (p-S496), AMPKalpha, AMPKa1, AMPK1, AMPK alpha 1, AMPK
Ig Type
IgG
Clone
3X970
Reactivity
Human
Verified Activity
1. Western blot analysis of Phospho-AMPK alpha 1(S496) on 293T cells lysates using anti-Phospho-AMPK alpha 1 (S496) antibody at 1/1,000 dilution.
2. ICC staining Phospho-AMPK alpha 1 (S496) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Phospho-AMPK alpha 1 (S496) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Phospho-AMPK alpha 1 (S496) in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:1000-5000; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundAMPK (5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. AMPKα1 (5'-AMP-activated protein kinase catalytic subunit alpha-1), also known as PRKAA1, is a 559 amino acid protein that belongs to the CAMK Ser/Thr protein kinase family and protein kinase superfamily. Highly phosphorylated, AMPKα1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 5p13.1.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
A synthesized phosphopeptide: human AMPK alpha 1 around the phosphorylation site of Ser496
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 64 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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