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Anti-Phospho-ALOX5 (Ser663) Polyclonal Antibody
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Catalog No. TMAB-10307
Anti-Phospho-ALOX5 (Ser663) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ALOX5 (Ser663). Anti-Phospho-ALOX5 (Ser663) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-ALOX5 (Ser663) Polyclonal Antibody
Anti-Phospho-ALOX5 (Ser663) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10307
Anti-Phospho-ALOX5 (Ser663) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ALOX5 (Ser663). Anti-Phospho-ALOX5 (Ser663) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-ALOX5 (Ser663) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ALOX5 (Ser663). Anti-Phospho-ALOX5 (Ser663) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Phospho-5-Lipoxygenase (Ser663) Polyclonal Antibody, Unconjugated (TMAB-10307) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: Heart (Rat) Lysate at 40 μg Primary: Anti-Phospho-5-Lipoxygenase (Ser663) (TMAB-10307) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 75 kD 3. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-5-Lipoxygenase (Ser663)) Polyclonal Antibody, Unconjugated (TMAB-10307) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-5-Lipoxygenase (Ser663)) Polyclonal Antibody, Unconjugated (TMAB-10307) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. |
| Immunogen | KLH conjugated synthesised phosphopeptide: rat 5-Lipoxygenase around the phosphorylation site of Ser663 |
| Antigen Species | Rat |
| Gene Name | ALOX5 |
| Gene ID | |
| Protein Name | Polyunsaturated fatty acid 5-lipoxygenase |
| Uniprot ID | |
| Function | Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. |
| Molecular Weight | Theoretical: 78 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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