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Anti-Phospho-AKT1 (Tyr326) Polyclonal Antibody
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Catalog No. TMAC-00111
Anti-Phospho-AKT1 (Tyr326) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Tyr326). Anti-Phospho-AKT1 (Tyr326) Polyclonal Antibody can be used in WB.
Anti-Phospho-AKT1 (Tyr326) Polyclonal Antibody
Anti-Phospho-AKT1 (Tyr326) Polyclonal Antibody
😃Good
Catalog No. TMAC-00111
Anti-Phospho-AKT1 (Tyr326) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Tyr326). Anti-Phospho-AKT1 (Tyr326) Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $216 | 7-10 days | |
| 100 μL | $316 | 7-10 days |
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| Description | Anti-Phospho-AKT1 (Tyr326) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Tyr326). Anti-Phospho-AKT1 (Tyr326) Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Application | |
| Recommended Dose | WB: 1:500-3000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 326 (N-D-Y(p)-G-R) derived from Human Akt |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 60 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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