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Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody
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Catalog No. TMAC-00115
Alias p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473)
Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Ser473). Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody can be used in IF,IHC,WB.
Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody
Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody
😃Good
Catalog No. TMAC-00115Alias p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473)
Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Ser473). Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody can be used in IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $218 | 7-10 days | |
| 100 μL | $317 | 7-10 days |
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| Description | Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Ser473). Anti-Phospho-AKT1 (Ser473) Polyclonal Antibody can be used in IF,IHC,WB. |
| Synonyms | p-AKT1 (Ser473), p-AKT1 (S473), AKT1 (p-Ser473), AKT1 (p-S473) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt(Phospho-Ser473) Antibody TMAC-00115(left) or the same antibody preincubated with blocking peptide(right). 2. Western blot analysis of extracts from SK-BR-3 cells,treated with insulin and EGF, and pretreated with U0126and LY294002 cells using Akt (Phospho-Ser473) AntibodyTMAC-00115. 3. Immunohistochemical analysis of paraffinembeddedhuman Lung carcinoma tissue usingAkt (Phospho-Ser473) Antibody TMAC-00115. 4. Western blot analysis of extracts of various cell lines, using Akt(Phospho-Ser473) TMAC-00115. 5. Western blot analysis of various lysates using Akt(Phospho-Ser473) Antibody at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (L3012) at 1:10000 dilution. Lysates/proteins: 25μg per lane. |
| Application | |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from Human Akt |
| Antigen Species | human |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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