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Anti-Phospho-AKT (Ser473) Antibody (8S643)
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Catalog No. TMAY-01580
Alias RAC-ALPHA, RAC, PRKBA, PKB-ALPHA, PKB, AKT
Anti-Phospho-AKT (Ser473) Antibody (8S643) is a Rabbit antibody targeting Phospho-AKT (Ser473). Anti-Phospho-AKT (Ser473) Antibody (8S643) can be used in WB,IHC-P.
Anti-Phospho-AKT (Ser473) Antibody (8S643)
Anti-Phospho-AKT (Ser473) Antibody (8S643)
😃Good
Catalog No. TMAY-01580Alias RAC-ALPHA, RAC, PRKBA, PKB-ALPHA, PKB, AKT
Anti-Phospho-AKT (Ser473) Antibody (8S643) is a Rabbit antibody targeting Phospho-AKT (Ser473). Anti-Phospho-AKT (Ser473) Antibody (8S643) can be used in WB,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $80 | 7-10 days | |
| 100 μL | $234 | 7-10 days |
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| Description | Anti-Phospho-AKT (Ser473) Antibody (8S643) is a Rabbit antibody targeting Phospho-AKT (Ser473). Anti-Phospho-AKT (Ser473) Antibody (8S643) can be used in WB,IHC-P. |
| Alias | RAC-ALPHA, RAC, PRKBA, PKB-ALPHA, PKB, AKT |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 8S643 |
| Reactivity | Human |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution. (Validation Experiment) 2. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution. 3. Western blot analysis of extracts from serum-starved NIH-3T3 treated with PDGFA (5 μg/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:1000, 1:50000, 1:200000 dilution. (Validation Experiment) 4. Western blot analysis of extracts from serum-starved A431, untreated (-); treated with EGF (200 ng/mL, 15 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower). (Validation Experiment) 5. Western blot analysis of extracts from serum-starved NIH-3T3, untreated (-); treated with PDGFA (5 μg/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower). 6. Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 μg/mL, 5 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution. (Validation Experiment) 7. Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5000 ng/mL, 5min; +) (line B); treated with PDGFA and λ-phosphatase (line C) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution. (Validation Experiment) |
| Application | WB,IHC-P |
| Recommended Dose | WB: 1:1000-1:200000; IHC-P: 1:100-1:400 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | The antibody is produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues around Ser473 of human AKT. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | v-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | A synthetic phosphopeptide corresponding to residues around Ser473 of human AKT. |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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