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Anti-Phospho-A Raf (Tyr301+Tyr302) Polyclonal Antibody

Catalog No. TMAB-10282 Copy Product Info
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Anti-Phospho-A Raf (Tyr301+Tyr302) Polyclonal Antibody is a Rabbit antibody targeting Phospho-A Raf (Tyr301+Tyr302). Anti-Phospho-A Raf (Tyr301+Tyr302) Polyclonal Antibody can be used in WB.

Anti-Phospho-A Raf (Tyr301+Tyr302) Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-10282

Anti-Phospho-A Raf (Tyr301+Tyr302) Polyclonal Antibody is a Rabbit antibody targeting Phospho-A Raf (Tyr301+Tyr302). Anti-Phospho-A Raf (Tyr301+Tyr302) Polyclonal Antibody can be used in WB.

Anti-Phospho-A Raf
(Tyr301+Tyr302) Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-A Raf (Tyr301+Tyr302) Polyclonal Antibody is a Rabbit antibody targeting Phospho-A Raf (Tyr301+Tyr302). Anti-Phospho-A Raf (Tyr301+Tyr302) Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
Sample: liver (Mouse) lysate at 30 μg;
Primary: Anti-Phospho-A Raf (Tyr301+Tyr302) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000 dilution;
Predicted band size: 67 kD
Observed band size: 67 kD
Application
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityPredominantly in urogenital tissues.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human A Raf around the phosphorylation site of Tyr301/302
Antigen Species
Human
Gene Name
ARAF
Gene ID
Protein Name
Serine/threonine-protein kinase A-Raf
Uniprot ID
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Chemical Properties
Molecular WeightTheoretical: 67 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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