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Anti-Phospho-A Raf (Ser214) Polyclonal Antibody

Catalog No. TMAB-10281 Copy Product Info
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Anti-Phospho-A Raf (Ser214) Polyclonal Antibody is a Rabbit antibody targeting Phospho-A Raf (Ser214). Anti-Phospho-A Raf (Ser214) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Phospho-A Raf (Ser214) Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-10281

Anti-Phospho-A Raf (Ser214) Polyclonal Antibody is a Rabbit antibody targeting Phospho-A Raf (Ser214). Anti-Phospho-A Raf (Ser214) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Phospho-A Raf
(Ser214) Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-A Raf (Ser214) Polyclonal Antibody is a Rabbit antibody targeting Phospho-A Raf (Ser214). Anti-Phospho-A Raf (Ser214) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-A Raf (Ser214)) Polyclonal Antibody, Unconjugated (TMAB-10281) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
IHC-P
IHC-Fr
IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityPredominantly in urogenital tissues.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human A Raf around the phosphorylation site of Ser214
Antigen Species
Human
Gene Name
ARAF
Gene ID
369
Protein Name
Serine/threonine-protein kinase A-Raf
Uniprot ID
P10398
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Chemical Properties
Molecular WeightTheoretical: 67 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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