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Anti-Phospho-4EBP1 (Ser65) Polyclonal Antibody (Synonyms: p-4EBP1 (Ser65), p-4EBP1 (S65), 4EBP1 (p-Ser65), 4E...)

Catalog No. TMAC-00016 Copy Product Info
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Anti-Phospho-4EBP1 (Ser65) Polyclonal Antibody is a Rabbit antibody targeting Phospho-4EBP1 (Ser65). Anti-Phospho-4EBP1 (Ser65) Polyclonal Antibody can be used in IHC,WB.

Anti-Phospho-4EBP1 (Ser65) Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAC-00016
Synonyms p-4EBP1 (Ser65), p-4EBP1 (S65), 4EBP1 (p-Ser65), 4EBP1 (p-S65)

Anti-Phospho-4EBP1 (Ser65) Polyclonal Antibody is a Rabbit antibody targeting Phospho-4EBP1 (Ser65). Anti-Phospho-4EBP1 (Ser65) Polyclonal Antibody can be used in IHC,WB.

Anti-Phospho-4EBP1
(Ser65) Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2187-10 days7-10 days
100 μL$3167-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-4EBP1 (Ser65) Polyclonal Antibody is a Rabbit antibody targeting Phospho-4EBP1 (Ser65). Anti-Phospho-4EBP1 (Ser65) Polyclonal Antibody can be used in IHC,WB.
Synonymsp-4EBP1 (Ser65), p-4EBP1 (S65), 4EBP1 (p-Ser65), 4EBP1 (p-S65)
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Phospho-Ser64) antibody TMAC-00016. The lane on the right is treated with the synthesized peptide.
2. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Ser64) antibody TMAC-00016. The picture on the right is treated with the synthesized peptide.
verifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:500-3000; IHC: 1:50-100
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
AppearanceLiquid
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundRegulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
Peptide sequence around phosphorylation site of serine 64 (R-N-S(p)-P-V) derived from Human 4E-BP1
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightActual: 15 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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