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Anti-PHLP Polyclonal Antibody

Catalog No. TMAB-10272 Copy Product Info
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Anti-PHLP Polyclonal Antibody is a Rabbit antibody targeting PHLP. Anti-PHLP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-PHLP Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-10272

Anti-PHLP Polyclonal Antibody is a Rabbit antibody targeting PHLP. Anti-PHLP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-PHLP Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-PHLP Polyclonal Antibody is a Rabbit antibody targeting PHLP. Anti-PHLP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Pig,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PHLP) Polyclonal Antibody, Unconjugated (TMAB-10272) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Rat Heart tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Rat Kidney tissue lysates
Lane 7: Human HUVEC cell lysates
Lane 8: Human K562 cell lysates
Primary: Anti-PHLP (TMAB-10272) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 40 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityExpressed in buccal mucosa cell and 236 other tissues
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundPhosducin-like protein is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PHLP
Antigen Species
Human
Gene Name
PDCL
Gene ID
Protein Name
Phosducin-like protein
Uniprot ID
Function
Acts as a positive regulator of hedgehog signaling and regulates ciliary function.Isoform 1:
Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers.Isoform 2:
Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin.
Chemical Properties
Molecular WeightTheoretical: 34 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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