Anti-PHF2 Polyclonal Antibody is a Rabbit antibody targeting PHF2. Anti-PHF2 Polyclonal Antibody can be used in IHC-P,WB,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (PHF2) Polyclonal Antibody, Unconjugated (TMAB-10266) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (PHF2) Polyclonal Antibody, Unconjugated (TMAB-10266) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample:
Lane 1: Recombinant human PHF2 protein, C-His
Primary: Anti-PHF2 (TMAB-10266) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kDa
Observed band size: 34 kDa

IHC-P: 1:100-500; WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

Recombinant Protein: human PHF2 protein

Human

PHF2

Lysine-specific demethylase PHF2

Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.