Anti-PGP9.5 Polyclonal Antibody is a Rabbit antibody targeting PGP9.5. Anti-PGP9.5 Polyclonal Antibody can be used in WB,ICC/IF.

IgG

Human,Mouse,Rat

1. 4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (PGP9.5) polyclonal Antibody, unconjugated (TMAB-10251) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 μg total protein per lane of various lysates (see on figure) probed with PGP9.5 polyclonal antibody, unconjugated (TMAB-10251) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; ICC/IF: 1:50-200

Polyclonal

Recombinant Protein: human UCHL1

Human

UCHL1

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.