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Anti-PGC Antibody (4S660)

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Catalog No. TMAB-10237

Anti-PGC Antibody (4S660) is a Mouse antibody targeting PGC. Anti-PGC Antibody (4S660) can be used in IHC-P,WB,IHC-Fr,IF.

Anti-PGC Antibody (4S660)

Anti-PGC Antibody (4S660)

Copy Product Info
😃Good
Catalog No. TMAB-10237
Anti-PGC Antibody (4S660) is a Mouse antibody targeting PGC. Anti-PGC Antibody (4S660) can be used in IHC-P,WB,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2227-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-PGC Antibody (4S660) is a Mouse antibody targeting PGC. Anti-PGC Antibody (4S660) can be used in IHC-P,WB,IHC-Fr,IF.
Ig Type
IgG2b,k
Clone
4S660
Reactivity
Human,Rat,Mouse
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PGC) Monoclonal Antibody, Unconjugated (TMAB-10237) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Sample:
Lane 1: Recombinant human PGC protein, C-His
Primary: Anti-PGC (TMAB-10237) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 40 kDa
3. Sample:
Lane 1: Mouse Stomach tissue lysates
Lane 2: Rat Stomach tissue lysates
Primary: Anti-PGC (TMAB-10237) at 1/1000 dilution
Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific
Predicted band size: 42 kDa
Observed band size: 39 kDa
Application
Recommended Dose
IHC-P: 1:100-500; WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationSecreted.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M PBS (pH7.4) with 0.02% Proclin300.
Research BackgroundThis gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Oct 2009]
Antigen Details
Immunogen
Recombinant Protein: human PGC protein
Antigen Species
Human
Gene Name
PGC
Gene ID
Protein Name
Gastricsin
Uniprot ID
Function
Hydrolyzes a variety of proteins.
Chemical Properties
Molecular WeightTheoretical: 42 kDa. Actual: 40 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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