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Anti-PFKM Antibody (4A746) (Synonyms: Phosphohexokinase, Phosphofructokinase 1, Phosphofructo-1-kinase isozyme...)

Catalog No. TMAC-01568 Copy Product Info
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Anti-PFKM Antibody (4A746) is a Rabbit antibody targeting PFKM. Anti-PFKM Antibody (4A746) can be used in FCM,ICC,IF,IHC,WB.

Anti-PFKM Antibody (4A746)

Copy Product Info
🥰Excellent
Catalog No. TMAC-01568
Synonyms Phosphohexokinase, Phosphofructokinase 1, Phosphofructo-1-kinase isozyme A, PFKX, PFKM, 6-phosphofructokinase, muscle type

Anti-PFKM Antibody (4A746) is a Rabbit antibody targeting PFKM. Anti-PFKM Antibody (4A746) can be used in FCM,ICC,IF,IHC,WB.

Anti-PFKM Antibody
(4A746)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2987-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-PFKM Antibody (4A746) is a Rabbit antibody targeting PFKM. Anti-PFKM Antibody (4A746) can be used in FCM,ICC,IF,IHC,WB.
SynonymsPhosphohexokinase, Phosphofructokinase 1, Phosphofructo-1-kinase isozyme A, PFKX, PFKM, 6-phosphofructokinase, muscle type
Ig Type
IgG
Clone
4A746
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of Fructose 6 Phosphate Kinase on PC-3M cell lysate using anti-Fructose 6 Phosphate Kinase antibody at 1/500 dilution.
2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
7. ICC staining Fructose 6 Phosphate Kinase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining Fructose 6 Phosphate Kinase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. ICC staining Fructose 6 Phosphate Kinase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
10. Flow cytometric analysis of SH-SY-5Y cells with Fructose 6 Phosphate Kinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundCatalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. In human PFK exists as a system of 3 types of subunits, PFKM (muscle), PFKL (liver) and PFKP (platelet) isoenzymes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 85 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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