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Anti-PFDN3 Polyclonal Antibody
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Catalog No. TMAB-10219
Anti-PFDN3 Polyclonal Antibody is a Rabbit antibody targeting PFDN3. Anti-PFDN3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PFDN3 Polyclonal Antibody
Anti-PFDN3 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10219
Anti-PFDN3 Polyclonal Antibody is a Rabbit antibody targeting PFDN3. Anti-PFDN3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-PFDN3 Polyclonal Antibody is a Rabbit antibody targeting PFDN3. Anti-PFDN3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFDN3) Polyclonal Antibody, Unconjugated (TMAB-10219) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: HepG2 (Human) Cell Lysate at 40 μg Raji (Human) Cell Lysate at 40 μg Primary: Anti-PFDN3 at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. In complex with VHL can translocate to the nucleus. |
| Tissue Specificity | Ubiquitous. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. The encoded protein functions as a chaperone protein, and may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. Alternative splicing and the use of alternate transcription start sites results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Jan 2015] |
| Immunogen | KLH conjugated synthetic peptide: human PFDN3 |
| Antigen Species | Human |
| Gene Name | VBP1 |
| Gene ID | |
| Protein Name | Prefoldin subunit 3 |
| Uniprot ID | |
| Function | Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. |
| Molecular Weight | Theoretical: 23 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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