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Anti-PFDN2 Antibody (5J899)

😃Good
Catalog No. TMAY-02721
Alias prefoldin subunit 2, PFD2

Anti-PFDN2 Antibody (5J899) is a Mouse antibody targeting PFDN2. Anti-PFDN2 Antibody (5J899) can be used in ICC/IF.

Anti-PFDN2 Antibody (5J899)

Anti-PFDN2 Antibody (5J899)

😃Good
Catalog No. TMAY-02721Alias prefoldin subunit 2, PFD2
Anti-PFDN2 Antibody (5J899) is a Mouse antibody targeting PFDN2. Anti-PFDN2 Antibody (5J899) can be used in ICC/IF.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-PFDN2 Antibody (5J899) is a Mouse antibody targeting PFDN2. Anti-PFDN2 Antibody (5J899) can be used in ICC/IF.
Aliasprefoldin subunit 2, PFD2
Ig Type
Monoclonal Mouse IgG1
Clone
5J899
Reactivity
Human
Verified Activity
Immunofluorescence staining of PFDN2 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human PFDN2 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor?594-conjugated Goat Anti-mouse IgG secondary antibody (red). Positive staining was localized to Nucleus and cytoplasm.
Application
ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PFDN2 (rh PFDN2; TMPY-03396; Q9UHV9; Met1-Ser154). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundPFD2, also known as PFDN2, belongs to the prefoldin beta subunit family. PFD2 is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. PFD2 binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. PFD2 also binds to a nascent polypeptide chain and promotes folding in a setting in which there are many competing pathways for nonnative proteins.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human PFDN2 Protein (TMPY-03396)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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