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Anti-PFDN2 Antibody (5J899)
😃Good
Catalog No. TMAY-02721
Alias prefoldin subunit 2, PFD2
Anti-PFDN2 Antibody (5J899) is a Mouse antibody targeting PFDN2. Anti-PFDN2 Antibody (5J899) can be used in ICC/IF.
Anti-PFDN2 Antibody (5J899)
Anti-PFDN2 Antibody (5J899)
😃Good
Catalog No. TMAY-02721Alias prefoldin subunit 2, PFD2
Anti-PFDN2 Antibody (5J899) is a Mouse antibody targeting PFDN2. Anti-PFDN2 Antibody (5J899) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ICC/IF Reactivity: Human |
| Alias | prefoldin subunit 2, PFD2 |
| Ig Type | Mouse IgG1 |
| Clone | 5J899 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of PFDN2 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human PFDN2 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor?594-conjugated Goat Anti-mouse IgG secondary antibody (red). Positive staining was localized to Nucleus and cytoplasm. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PFDN2 (rh PFDN2; TMPY-03396; Q9UHV9; Met1-Ser154). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | PFD2, also known as PFDN2, belongs to the prefoldin beta subunit family. PFD2 is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. PFD2 binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. PFD2 also binds to a nascent polypeptide chain and promotes folding in a setting in which there are many competing pathways for nonnative proteins. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human PFDN2 Protein (TMPY-03396) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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