Anti-Peroxiredoxin-6 Antibody (6O984) is a Rabbit antibody targeting Peroxiredoxin-6. Anti-Peroxiredoxin-6 Antibody (6O984) can be used in WB, ICC/IF, IP, FCM.

IgG

6O984

Human,Mouse,Rat

1. The A431 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-Peroxiredoxin-6 antibody (TMAB-10205, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed A431 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Peroxiredoxin-6) monoclonal Antibody, unconjugated (TMAB-10205) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. 25 ug total protein per lane of various lysates (see on figure) probed with Peroxiredoxin-6 monoclonal antibody, unconjugated (TMAB-10205) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human PRDX6

Human

PRDX6

Peroxiredoxin-6

Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.