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Anti-Peroxiredoxin 5 Antibody (6B923)
🥰Excellent
Catalog No. TMAY-01803
Anti-Peroxiredoxin 5 Antibody (6B923) is a Rabbit antibody targeting Peroxiredoxin 5. Anti-Peroxiredoxin 5 Antibody (6B923) can be used in WB,ICC/IF,IP.
Anti-Peroxiredoxin 5 Antibody (6B923)
Anti-Peroxiredoxin 5 Antibody (6B923)
🥰Excellent
Catalog No. TMAY-01803
Anti-Peroxiredoxin 5 Antibody (6B923) is a Rabbit antibody targeting Peroxiredoxin 5. Anti-Peroxiredoxin 5 Antibody (6B923) can be used in WB,ICC/IF,IP.
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Chemical Properties
| Description | Anti-Peroxiredoxin 5 Antibody (6B923) is a Rabbit antibody targeting Peroxiredoxin 5. Anti-Peroxiredoxin 5 Antibody (6B923) can be used in WB,ICC/IF,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6B923 |
| Reactivity | Human |
| Verified Activity | 1. PRDX5 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg Jurkat Whole Cell Lysate. -Lane C:0.5 mg 293T Whole Cell Lysate. -2 µL anti-PRDX5 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PRDX5 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 22 kDa. -Observed band size: 22 kDa. 2. Anti-PRDX5 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: 293T Whole Cell Lysate. -Lane C: Jurkat Whole Cell Lysate. -Lane D: 293 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:22 kDa. -Observed band size:15 kDa. 3. Immunofluorescence staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PRDX5 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). 4. Anti-PRDX5 rabbit monoclonal antibody at 1:1000 dilution. -Lane A: PRDX5 konckout Hela Whole Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:22 kDa. -Observed band size:15 kDa(Validation Experiment) |
| Application | WB,ICC/IF,IP |
| Recommended Dose | WB: 1:500-1:2000; ICC-IF: 1:20-1:100; IP: 4-8 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Peroxiredoxin 5 / PRDX5 (rh Peroxiredoxin 5 / PRDX5; TMPY-02208; P30044-2; Met53-Leu214). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Peroxiredoxin-5, also known as Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, Peroxisomal antioxidant enzyme, Thioredoxin peroxidase PMP20, Thioredoxin reductase, PRDX5 and ACR1, is cytoplasm protein that belongs to the peroxiredoxin 2 family. Peroxiredoxin-5 / PRDX5 reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-5 / PRDX5 is involved in intracellular redox signaling. The Peroxiredoxins / Prx are a family of 25 kDa peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins / Prx family is divided into six groups ( PRDX1，PRDX2, PRDX3, PRDX4, PRDX5, PRDX6 ) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins / Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins / Prx has a proliferative effect and may be related to cancer development or progression. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Peroxiredoxin 5 / PRDX5 protein (TMPY-02208) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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