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Anti-Peroxiredoxin 1 Antibody (9D82)

😃Good
Catalog No. TMAC-03164
Alias TDPX2, PRXI, PRX1, peroxiredoxin 1, PAGB, PAGA, PAG, NKEF-A, NKEFA, MSP23

Anti-Peroxiredoxin 1 Antibody (9D82) is a Rabbit antibody targeting Peroxiredoxin 1. Anti-Peroxiredoxin 1 Antibody (9D82) can be used in WB,ICC/IF,IHC,FCM.

Anti-Peroxiredoxin 1 Antibody (9D82)

Anti-Peroxiredoxin 1 Antibody (9D82)

😃Good
Catalog No. TMAC-03164Alias TDPX2, PRXI, PRX1, peroxiredoxin 1, PAGB, PAGA, PAG, NKEF-A, NKEFA, MSP23
Anti-Peroxiredoxin 1 Antibody (9D82) is a Rabbit antibody targeting Peroxiredoxin 1. Anti-Peroxiredoxin 1 Antibody (9D82) can be used in WB,ICC/IF,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2967-10 days
100 μL$4967-10 days
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Product Introduction

Bioactivity
Description
Anti-Peroxiredoxin 1 Antibody (9D82) is a Rabbit antibody targeting Peroxiredoxin 1. Anti-Peroxiredoxin 1 Antibody (9D82) can be used in WB,ICC/IF,IHC,FCM.
SynonymsTDPX2, PRXI, PRX1, peroxiredoxin 1, PAGB, PAGA, PAG, NKEF-A, NKEFA, MSP23
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Western blot analysis of Peroxiredoxin 1 on different lysates using anti-Peroxiredoxin 1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: A431.
2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Peroxiredoxin 1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Peroxiredoxin 1 antibody. Counter stained with hematoxylin.
4. ICC staining Peroxiredoxin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Peroxiredoxin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of Hela cells with Peroxiredoxin 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis. The human PRX IV gene is abundantly expressed in many tissues.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 22 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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