Anti-Peroxiredoxin 1 Antibody (5C204) is a Rabbit antibody targeting Peroxiredoxin 1. Anti-Peroxiredoxin 1 Antibody (5C204) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

5C204

Human,Mouse

1. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Peroxiredoxin 1) Polyclonal Antibody, Unconjugated (TMAB-10197) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
HepG2 (Human) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
U2Os (Human) Cell Lysate at 30 μg
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-Peroxiredoxin 1 (TMAB-10197) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD

WB: 1:500-2000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000

Monoclonal

Recombinant Protein: human Peroxiredoxin 1 protein, full length

Human

PRDX1

Peroxiredoxin-1

Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.