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Anti-Periostin Antibody (2Y740)
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Catalog No. TMAB-10191
Anti-Periostin Antibody (2Y740) is a Rabbit antibody targeting Periostin. Anti-Periostin Antibody (2Y740) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-Periostin Antibody (2Y740)
Anti-Periostin Antibody (2Y740)
Copy Product Info😃Good
Catalog No. TMAB-10191
Anti-Periostin Antibody (2Y740) is a Rabbit antibody targeting Periostin. Anti-Periostin Antibody (2Y740) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
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| Description | Anti-Periostin Antibody (2Y740) is a Rabbit antibody targeting Periostin. Anti-Periostin Antibody (2Y740) can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Clone | 2Y740 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Periostin on human brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST in PBS for 1 hour at room temperature. The primary antibody (TMAB-10191, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1: 200,000 dilution was used for 1 hour at room temperature. Predicted band size: 93 kDa Observed band size: 80 kDa 2. Flow cytometric analysis of Periostin was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (TMAB-10191, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). 3. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated (TMAB-10191) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated (TMAB-10191) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated (TMAB-10191) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated (TMAB-10191) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated (TMAB-10191) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:20-50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Tissue Specificity | Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Periostin is a disulfide linked 90 kDa, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta 1(TGF beta 1) and bone morphogenetic protein (BMP2). Periostin has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart. Abnormal expression of periostin is also linked to angiogenesis and metastatsis in epithelial tumors. |
| Immunogen | A synthesized peptide: human Periostin |
| Antigen Species | Human |
| Gene Name | POSTN |
| Gene ID | |
| Protein Name | Periostin |
| Uniprot ID | |
| Function | Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. |
| Molecular Weight | Theoretical: 100 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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