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Anti-Perilipin A Polyclonal Antibody
😃Good
Catalog No. TMAB-01369
Alias PLIN1, PLIN 1, PLIN, Perilipin-A, PerilipinA, Perilipin1, Perilipin 1, Perilipin, PERI, Lipid droplet associated protein
Anti-Perilipin A Polyclonal Antibody is a Rabbit antibody targeting Perilipin A. Anti-Perilipin A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Perilipin A Polyclonal Antibody
Anti-Perilipin A Polyclonal Antibody
😃Good
Catalog No. TMAB-01369Alias PLIN1, PLIN 1, PLIN, Perilipin-A, PerilipinA, Perilipin1, Perilipin 1, Perilipin, PERI, Lipid droplet associated protein
Anti-Perilipin A Polyclonal Antibody is a Rabbit antibody targeting Perilipin A. Anti-Perilipin A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Perilipin A Polyclonal Antibody is a Rabbit antibody targeting Perilipin A. Anti-Perilipin A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | PLIN1, PLIN 1, PLIN, Perilipin-A, PerilipinA, Perilipin1, Perilipin 1, Perilipin, PERI, Lipid droplet associated protein |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Sample: Parotid gland (Mouse) Lysate at 40 μg Primary: Anti-Perilipin A (TMAB-01369) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa 2. Sample: Thymus (Mouse) Lysate at 40 μg Primary: Anti-Perilipin A (TMAB-01369) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Perilipin A) Polyclonal Antibody, Unconjugated (TMAB-01369) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining. 4. Sample: MCF-7 Cell (Human) Lysate at 30 μg Primary: Anti-Perilipin A at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Lipid droplet. Note=Lipid droplet surface-associated. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Perilipins, members of the PAT protein family (named after lipid droplet proteins Perilipin, Adipophilin, and TIP47) are found exclusively at the surface of lipid droplets in adipocytes and steroidogenic cells. They have been suggested to function as regulators of lipolysis and triacylglycerol storage within adipose tissue. Four distinct isoforms ranging from perilipin A (57 kDa) to perilipin D (26 kDa) have been identified and they share an identical amino terminal sequences, and contain 2–6 consensus protein kinase A (PKA) phosphorylation sites. Perilipin C and D have been detected only in steroidogenic cells. Perilipin A is the most abundant form on the lipid droplets of adipocytes. The phosphorylation of perilipin by PKA, which is accompanied by the phosphorylation and translocation of hormone-sensitive lipase from the cytosol to the lipid droplets, promotes lipolysis. There is evidence for the presence of perilipin A in atheroma plaques suggesting that the protein may be involved in the development of therosclerosis by controlling as in adipocytes the hydrolysis of stored lipids. |
| Immunogen | KLH conjugated synthetic peptide: human Perilipin-1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Metabolism of lipids and lipoproteins,Lipoprotein metabolism,Lipid Droplet Protein,Lipid metabolism,Lipoprotein metabolism,Heart disease,Lipid metabolism |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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