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Anti-Perilipin A+B Polyclonal Antibody
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Catalog No. TMAB-10189
Anti-Perilipin A+B Polyclonal Antibody is a Rabbit antibody targeting Perilipin A+B. Anti-Perilipin A+B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-Perilipin A+B Polyclonal Antibody
Anti-Perilipin A+B Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10189
Anti-Perilipin A+B Polyclonal Antibody is a Rabbit antibody targeting Perilipin A+B. Anti-Perilipin A+B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Perilipin A+B Polyclonal Antibody is a Rabbit antibody targeting Perilipin A+B. Anti-Perilipin A+B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Chicken,Dog,Sheep) |
| Verified Activity | 1. Blank control: MCF7 (blue). Primary Antibody: Rabbit Anti-Perilipin A+B/AF647 Conjugated antibody (TMAB-10189-AF647), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions. 2. Blank control: U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Perilipin A+B antibody (TMAB-10189), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA. 3. This image was generously provided by James Fox, PhD. at the University of York. Immunostaining of perilipin-positive adipocytes (red) using Rabbit Anti-Perilipin A+B Polyclonal Antibody (TMAB-10189) in bone marrow of IL-7cre Rosa26-EYFP mice with DAPI (blue) nuclear counterstain. 4. Sample: Stomach (Mouse) Lysate at 40 μg Primary: Anti-Perilipin A+B (TMAB-10189) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54-56 kD Observed band size: 56 kD 5. Sample: HepG2 Cell (Human) Lysate at 30 μg Primary: Anti-Perilipin A+B (TMAB-10189) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54-56 kD Observed band size: 50 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Lipid droplet. Note=Lipid droplet surface-associated. |
| Tissue Specificity | Adipocytes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Perilipins, members of the PAT protein family (named after lipid droplet proteins Perilipin, Adipophilin, and TIP47) are found exclusively at the surface of lipid droplets in adipocytes and steroidogenic cells. They have been suggested to function as regulators of lipolysis and triacylglycerol storage within adipose tissue. Four distinct isoforms ranging from perilipin A (57 kDa) to perilipin D (26 kDa) have been identified and they share an identical amino terminal sequences, and contain 2–6 consensus protein kinase A (PKA) phosphorylation sites. Perilipin C and D have been detected only in steroidogenic cells. Perilipin A is the most abundant form on the lipid droplets of adipocytes. The phosphorylation of perilipin by PKA, which is accompanied by the phosphorylation and translocation of hormone-sensitive lipase from the cytosol to the lipid droplets, promotes lipolysis. There is evidence for the presence of perilipin A in atheroma plaques suggesting that the protein may be involved in the development of therosclerosis by controlling as in adipocytes the hydrolysis of stored lipids. |
| Immunogen | KLH conjugated synthetic peptide: human Perilipin A+B |
| Antigen Species | Human |
| Gene Name | PLIN1 |
| Gene ID | |
| Protein Name | Perilipin |
| Uniprot ID | |
| Function | Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. |
| Molecular Weight | Theoretical: 54-56 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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