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Anti-Perilipin A Antibody (3V1)
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Catalog No. TMAB-10188
Anti-Perilipin A Antibody (3V1) is a Rabbit antibody targeting Perilipin A. Anti-Perilipin A Antibody (3V1) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Perilipin A Antibody (3V1)
Anti-Perilipin A Antibody (3V1)
Copy Product Info😃Good
Catalog No. TMAB-10188
Anti-Perilipin A Antibody (3V1) is a Rabbit antibody targeting Perilipin A. Anti-Perilipin A Antibody (3V1) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Perilipin A Antibody (3V1) is a Rabbit antibody targeting Perilipin A. Anti-Perilipin A Antibody (3V1) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 3V1 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Rat Adipose; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Perilipin A Monoclonal Antibody, Unconjugated (TMAB-10188) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Perilipin A Monoclonal Antibody, Unconjugated (TMAB-10188) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. 25 μg total protein per lane of various lysates (see on figure) probed with Perilipin A monoclonal antibody, unconjugated (TMAB-10188) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 4. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Perilipin A Monoclonal Antibody, Unconjugated (TMAB-10188) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei. 5. Paraformaldehyde-fixed, paraffin embedded Human fat; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Perilipin A Monoclonal Antibody, Unconjugated (TMAB-10188) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:50-100; IHC-Fr: 1:50-100; IF: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Lipid droplet. Note=Lipid droplet surface-associated. |
| Tissue Specificity | Adipocytes. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Perilipins, members of the PAT protein family (named after lipid droplet proteins Perilipin, Adipophilin, and TIP47) are found exclusively at the surface of lipid droplets in adipocytes and steroidogenic cells. They have been suggested to function as regulators of lipolysis and triacylglycerol storage within adipose tissue. Four distinct isoforms ranging from perilipin A (57 kDa) to perilipin D (26 kDa) have been identified and they share an identical amino terminal sequences, and contain 2–6 consensus protein kinase A (PKA) phosphorylation sites. Perilipin C and D have been detected only in steroidogenic cells. Perilipin A is the most abundant form on the lipid droplets of adipocytes. The phosphorylation of perilipin by PKA, which is accompanied by the phosphorylation and translocation of hormone-sensitive lipase from the cytosol to the lipid droplets, promotes lipolysis. There is evidence for the presence of perilipin A in atheroma plaques suggesting that the protein may be involved in the development of therosclerosis by controlling as in adipocytes the hydrolysis of stored lipids. |
| Immunogen | A synthesized peptide: human Perilipin 1 |
| Antigen Species | Human |
| Gene Name | PLIN1 |
| Gene ID | |
| Protein Name | Perilipin |
| Uniprot ID | |
| Function | Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. |
| Molecular Weight | Theoretical: 57 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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