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Anti-PER1 Polyclonal Antibody

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Catalog No. TMAB-01368
Alias RIGUI, Period1, Period homolog 1, Period drosophila homolog of, Period circadian protein homolog 1, Period 1, PER1, PER 1, PER, MGC88021, KIAA0482, hPER1, hPER 1, hPER, Circadian pacemaker protein Rigui, Circadian clock protein PERIOD1, Circadian clock protein PERIOD 1

Anti-PER1 Polyclonal Antibody is a Rabbit antibody targeting PER1. Anti-PER1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-PER1 Polyclonal Antibody

Anti-PER1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01368Alias RIGUI, Period1, Period homolog 1, Period drosophila homolog of, Period circadian protein homolog 1, Period 1, PER1, PER 1, PER, MGC88021, KIAA0482, hPER1, hPER 1, hPER, Circadian pacemaker protein Rigui, Circadian clock protein PERIOD1, Circadian clock protein PERIOD 1
Anti-PER1 Polyclonal Antibody is a Rabbit antibody targeting PER1. Anti-PER1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-PER1 Polyclonal Antibody is a Rabbit antibody targeting PER1. Anti-PER1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasRIGUI, Period1, Period homolog 1, Period drosophila homolog of, Period circadian protein homolog 1, Period 1, PER1, PER 1, PER, MGC88021, KIAA0482, hPER1, hPER 1, hPER, Circadian pacemaker protein Rigui, Circadian clock protein PERIOD1, Circadian clock protein PERIOD 1
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PER1 protein) Polyclonal Antibody, Unconjugated (TMAB-01368) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human A549 cell lysates
Primary: Anti-PER1 (TMAB-01368) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 136 kDa
Observed band size: 142 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus (By similarity). Cytoplasm (By similarity). Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2 (By similarity).
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundIn mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT 3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila PER. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL 1 transactivation complex.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PER1 protein
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
HLH,Transcription Factors,Circadian Rhythm
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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