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Anti-PER1 Polyclonal Antibody
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Catalog No. TMAB-01368
Alias RIGUI, Period1, Period homolog 1, Period drosophila homolog of, Period circadian protein homolog 1, Period 1, PER1, PER 1, PER, MGC88021, KIAA0482, hPER1, hPER 1, hPER, Circadian pacemaker protein Rigui, Circadian clock protein PERIOD1, Circadian clock protein PERIOD 1
Anti-PER1 Polyclonal Antibody is a Rabbit antibody targeting PER1. Anti-PER1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PER1 Polyclonal Antibody
Anti-PER1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01368Alias RIGUI, Period1, Period homolog 1, Period drosophila homolog of, Period circadian protein homolog 1, Period 1, PER1, PER 1, PER, MGC88021, KIAA0482, hPER1, hPER 1, hPER, Circadian pacemaker protein Rigui, Circadian clock protein PERIOD1, Circadian clock protein PERIOD 1
Anti-PER1 Polyclonal Antibody is a Rabbit antibody targeting PER1. Anti-PER1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PER1 Polyclonal Antibody is a Rabbit antibody targeting PER1. Anti-PER1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | RIGUI, Period1, Period homolog 1, Period drosophila homolog of, Period circadian protein homolog 1, Period 1, PER1, PER 1, PER, MGC88021, KIAA0482, hPER1, hPER 1, hPER, Circadian pacemaker protein Rigui, Circadian clock protein PERIOD1, Circadian clock protein PERIOD 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PER1 protein) Polyclonal Antibody, Unconjugated (TMAB-01368) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human A549 cell lysates Primary: Anti-PER1 (TMAB-01368) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 136 kDa Observed band size: 142 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus (By similarity). Cytoplasm (By similarity). Note=Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2 (By similarity). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT 3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila PER. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL 1 transactivation complex. |
| Immunogen | KLH conjugated synthetic peptide: human PER1 protein |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | HLH,Transcription Factors,Circadian Rhythm |
| Molecular Weight | Theoretical: 136 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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