Anti-PENK Polyclonal Antibody 2 is a Rabbit antibody targeting PENK. Anti-PENK Polyclonal Antibody 2 can be used in FCM.

IgG

Human (predicted:Mouse,Rat)

Blank control (black line): sH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Met Enkephalin antibody (TMAB-10171)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated Met Enkephalin peptide (Tyr-Gly-Gly-Phe-Met)

PENK

Proenkephalin-A

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.