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Anti-PEF1 Polyclonal Antibody is a Rabbit antibody targeting PEF1. Anti-PEF1 Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-PEF1 Polyclonal Antibody is a Rabbit antibody targeting PEF1. Anti-PEF1 Polyclonal Antibody can be used in WB,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Pig,Cow,Horse,Sheep,Monkey) |
| Verified Activity | 1. Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange); Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA; Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green). 2. Sample: Intestine (Mouse) Lysate at 40 μg Primary: Anti-PEF1 (TMAB-10162) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | PEF1 (penta-EF hand domain containing 1) is a Ca(2+)-binding protein that belongs to the penta-EF hand family which shares similarities to the apoptosis linked gene ALG-2. PEF1 heterodimerizes with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca(2+). |
| Immunogen | KLH conjugated synthetic peptide: human PEF1 |
| Antigen Species | Human |
| Gene Name | PEF1 |
| Gene ID | |
| Protein Name | Peflin |
| Uniprot ID |
| Molecular Weight | Theoretical: 30 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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