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Anti-PEF1 Polyclonal Antibody

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Catalog No. TMAB-10162

Anti-PEF1 Polyclonal Antibody is a Rabbit antibody targeting PEF1. Anti-PEF1 Polyclonal Antibody can be used in WB,FCM.

Anti-PEF1 Polyclonal Antibody

Anti-PEF1 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-10162
Anti-PEF1 Polyclonal Antibody is a Rabbit antibody targeting PEF1. Anti-PEF1 Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-PEF1 Polyclonal Antibody is a Rabbit antibody targeting PEF1. Anti-PEF1 Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Pig,Cow,Horse,Sheep,Monkey)
Verified Activity
1. Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG (orange);
Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue),
Dilution: 1: 200 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green).
2. Sample: Intestine (Mouse) Lysate at 40 μg
Primary: Anti-PEF1 (TMAB-10162) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Application
Recommended Dose
WB: 1:500-2000; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated in the presence of Ca(2+).
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundPEF1 (penta-EF hand domain containing 1) is a Ca(2+)-binding protein that belongs to the penta-EF hand family which shares similarities to the apoptosis linked gene ALG-2. PEF1 heterodimerizes with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca(2+).
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PEF1
Antigen Species
Human
Gene Name
PEF1
Gene ID
Protein Name
Peflin
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 30 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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