Anti-PDXK Polyclonal Antibody is a Rabbit antibody targeting PDXK. Anti-PDXK Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Horse)

1. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-PDXK antibody (TMAB-10154)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-PDXK antibody (TMAB-10154)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human PDXK

Human

PDXK

Pyridoxal kinase

Required for synthesis of pyridoxal-5-phosphate from vitamin B6.