Anti-PDIA6 Antibody (2X17) is a Rabbit antibody targeting PDIA6. Anti-PDIA6 Antibody (2X17) can be used in WB,ICC/IF,IHC-P,IHC-Fr,IF.

IgG

2X17

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PDIA6 Monoclonal Antibody, Unconjugated (TMAB-10135) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PDIA6 Monoclonal Antibody, Unconjugated (TMAB-10135) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PDIA6 Monoclonal Antibody, Unconjugated (TMAB-10135) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PDIA6 Monoclonal Antibody, Unconjugated (TMAB-10135) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PDIA6 Monoclonal Antibody, Unconjugated (TMAB-10135) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. 25 μg total protein per lane of various lysates (see on figure) probed with PDIA6 monoclonal antibody, unconjugated (TMAB-10135) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
7. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (PDIA6) monoclonal Antibody, unconjugated (TMAB-10135) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; ICC/IF: 1:50-200; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

A synthesized peptide: human PDIA6

Human

PDIA6

Protein disulfide-isomerase A6

May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.