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Anti-PDHE1 Antibody (8V568)
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Catalog No. TMAB-10128 Copy Product Info
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Anti-PDHE1 Antibody (8V568) is a Mouse antibody targeting PDHE1. Anti-PDHE1 Antibody (8V568) can be used in WB,ICC/IF.
Anti-PDHE1 Antibody
(8V568)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 25 µL | $149 | 7-10 days | 7-10 days | |
| 50 µL | $262 | 7-10 days | 7-10 days | |
| 100 µL | $471 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Stability & Storage
| Description | Anti-PDHE1 Antibody (8V568) is a Mouse antibody targeting PDHE1. Anti-PDHE1 Antibody (8V568) can be used in WB,ICC/IF. |
| Ig Type | IgG2a, Kappa |
| Clone | 8V568 |
| Reactivity | Human |
| Verified Activity | 1. Cell line: HepG2 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for TMAB-10128 2. Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4°C overnight Lysate: 1: HeLa, 2: HEK-293, 3: MCF-7, 4: Jurkat Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 43 kDa Observed MW: 43 kDa |
| Application | |
| Recommended Dose | WB: 1:500-1000; ICC/IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Mitochondrion matrix. |
| Tissue Specificity | Testis. Expressed in postmeiotic spermatogenic cells. |
| Construction | Recombinant Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase(E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Gene Name | PDHA1 |
| Protein Name | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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