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Anti-PDHA1 Antibody (9B780)
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Catalog No. TMAC-03129
Anti-PDHA1 Antibody (9B780) is a Rabbit antibody targeting PDHA1. Anti-PDHA1 Antibody (9B780) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-PDHA1 Antibody (9B780)
Anti-PDHA1 Antibody (9B780)
😃Good
Catalog No. TMAC-03129
Anti-PDHA1 Antibody (9B780) is a Rabbit antibody targeting PDHA1. Anti-PDHA1 Antibody (9B780) can be used in FCM,ICC/IF,IHC,IP,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $298 | 7-10 days | 7-10 days | |
| 100 μL | $498 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PDHA1 Antibody (9B780) is a Rabbit antibody targeting PDHA1. Anti-PDHA1 Antibody (9B780) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Ig Type | IgG |
| Clone | 9B780 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PDHA1 on different lysates using anti-PDHA1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T, Lane 2: A431, Lane 3: Mouse heart. 2. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PDHA1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDHA1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDHA1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-PDHA1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PDHA1 antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PDHA1 antibody. Counter stained with hematoxylin. 8. ICC staining PDHA1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining PDHA1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining PDHA1 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 11. Flow cytometric analysis of Hela cells with PDHA1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.            |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial matrix enzyme complex that functions as the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The E1 enzyme of the PDH complex is made up of a heterotetramer of two α and two β subunits. The E1-α subunit (PDH-E1α) contains the E1 active site and plays a key role in the function of the PDH complex. The PDH complex is regulated by phosphorylation and dephosphorylation of PDH-E1α. The gene encoding for PDH-E1α maps to chromosome Xp22.12, and a 20bp deletion in the last exon of this gene is sufficient to cause PDH deficiency, which causes a broad range of symptoms including the development of seizures, mental retardation and spasticity, as well as intermittent episodes of lactic acidosis associated with cerebellar ataxia. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 43 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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