Anti-PDGF-BB Polyclonal Antibody is a Rabbit antibody targeting PDGF-BB. Anti-PDGF-BB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Cow,Horse,Rabbit,Sheep)

1. Tissue/cell: rat transplant lymphoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PDGF-B Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Rat) Lysate at 40 μg
Lane 4: Lung (Mouse) Lysate at 40 μg
Lane 5: Lung (Rat) Lysate at 40 μg
Lane 6: Huvec (Human) Cell Lysate at 30 μg
Lane 7: A431 (Human) Cell Lysate at 30 μg
Lane 8: MCF-7 (Human) Cell Lysate at 30 μg
Lane 9: U2os (Human) Cell Lysate at 30 μg
Primary: Anti-PDGF-B (TMAB-01352) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 28 kDa
3. Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-PDGF-B antibody (TMAB-01352)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): f (ab/)2 fragment goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Sample:
Stomach (Rat) Lysate at 40 μg
Primary: Anti-PDGF-B (TMAB-01352) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human PDGF-B

Human

PDGFB

Platelet-derived growth factor subunit B

Response to hypoxia,PDGF,Inflammatory mediators,Serum Proteins,Angiogenesis and vasculogenesis,Hypoxia,Heart disease,PDGF

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.