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Anti-PDGF-BB Polyclonal Antibody is a Rabbit antibody targeting PDGF-BB. Anti-PDGF-BB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-PDGF-BB Polyclonal Antibody is a Rabbit antibody targeting PDGF-BB. Anti-PDGF-BB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Alias | PDGF-BB, PDGFBB |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Dog,Cow,Horse,Rabbit,Sheep) |
Verified Activity | 1. Tissue/cell: rat transplant lymphoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-PDGF-B Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Kidney (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Cerebrum (Rat) Lysate at 40 μg Lane 4: Lung (Mouse) Lysate at 40 μg Lane 5: Lung (Rat) Lysate at 40 μg Lane 6: Huvec (Human) Cell Lysate at 30 μg Lane 7: A431 (Human) Cell Lysate at 30 μg Lane 8: MCF-7 (Human) Cell Lysate at 30 μg Lane 9: U2os (Human) Cell Lysate at 30 μg Primary: Anti-PDGF-B (TMAB-01352) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25 kDa Observed band size: 28 kDa 3. Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-PDGF-B antibody (TMAB-01352) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): f (ab/)2 fragment goat anti-rabbit IgG-FITC Dilution: 1 μg/test. Protocol The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. 4. Sample: Stomach (Rat) Lysate at 40 μg Primary: Anti-PDGF-B (TMAB-01352) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 32 kDa |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted. Note=Released by platelets upon wounding. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heal the wound. [SUBUNIT] Antiparallel disulfide-linked dimer of nonidentical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. Belongs to the PDGF/VEGF growth factor family. |
Immunogen | KLH conjugated synthetic peptide: human PDGF-B |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Response to hypoxia,PDGF,Inflammatory mediators,Serum Proteins,Angiogenesis and vasculogenesis,Hypoxia,Heart disease,PDGF |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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