Anti-PDCD1LG2 Polyclonal Antibody is a Rabbit antibody targeting PDCD1LG2. Anti-PDCD1LG2 Polyclonal Antibody can be used in ICC/IF,FCM.

IgG

Human (predicted:Mouse,Rat,Cow)

1. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PDCD1LG2) polyclonal Antibody, Unconjugated (TMAB-10097) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
2. Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-PDCD1LG2 antibody (TMAB-10097)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

ICC/IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human PDCD1LG2

Human

PDCD1LG2

Programmed cell death 1 ligand 2

Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).