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Anti-PD-1 Polyclonal Antibody

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Catalog No. TMAB-01347
Alias SLEB2, programmed cell death 1, PD-1, PD1, hSLE1, hPD-l, hPD-1, CD279

Anti-PD-1 Polyclonal Antibody is a Rabbit antibody targeting PD-1. Anti-PD-1 Polyclonal Antibody can be used in FCM.

Anti-PD-1 Polyclonal Antibody

Anti-PD-1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01347Alias SLEB2, programmed cell death 1, PD-1, PD1, hSLE1, hPD-l, hPD-1, CD279
Anti-PD-1 Polyclonal Antibody is a Rabbit antibody targeting PD-1. Anti-PD-1 Polyclonal Antibody can be used in FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-PD-1 Polyclonal Antibody is a Rabbit antibody targeting PD-1. Anti-PD-1 Polyclonal Antibody can be used in FCM.
SynonymsSLEB2, programmed cell death 1, PD-1, PD1, hSLE1, hPD-l, hPD-1, CD279
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
Blank control (blue line): Mouse spleen cells (blue).
Primary Antibody (green line): Rabbit Anti-PD-1/PE-CY7 Conjugated antibody (TMAB-01347-PE-CY7)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7. Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4°C. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.
Application
Recommended Dose
FCM: 1μg /test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
Tissue SpecificityExpressed in lymph node and 85 other tissues
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundProgrammed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PD-1
Antigen Species
Human
Gene Name
PDCD1
Gene ID
Protein Name
Programmed cell death protein 1
Uniprot ID
Biology Area
CD,Regulatory T Cells,Death Receptors,CD markers,Apoptosis,Apoptosis
Function
Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005).Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005).Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity).Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity).The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311).The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311).The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).
Chemical Properties
Molecular WeightTheoretical: 32 kDa. Actual: 55-60 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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