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Anti-PCYT1A Antibody (4E85) is a Rabbit antibody targeting PCYT1A. Anti-PCYT1A Antibody (4E85) can be used in WB,ICC/IF,IP,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
| Description | Anti-PCYT1A Antibody (4E85) is a Rabbit antibody targeting PCYT1A. Anti-PCYT1A Antibody (4E85) can be used in WB,ICC/IF,IP,FCM. |
| Ig Type | IgG |
| Clone | 4E85 |
| Reactivity | Human |
| Verified Activity | 1. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (PCYT1A) monoclonal Antibody, unconjugated (TMAB-10091) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 2. 25 μg total protein per lane of various lysates (see on figure) probed with PCYT1A monoclonal antibody, unconjugated (TMAB-10091) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm; cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis. |
| Immunogen | A synthesized peptide: human PCYT1A |
| Antigen Species | Human |
| Gene Name | PCYT1A |
| Gene ID | |
| Protein Name | Choline-phosphate cytidylyltransferase A |
| Uniprot ID | |
| Function | Controls phosphatidylcholine synthesis. |
| Molecular Weight | Theoretical: 42 kDa. Actual: 42 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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