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Anti-PCNA Antibody (2C751)
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Catalog No. TMAC-03101
Alias proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (2C751) is a Rabbit antibody targeting PCNA. Anti-PCNA Antibody (2C751) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-PCNA Antibody (2C751)
Anti-PCNA Antibody (2C751)
😃Good
Catalog No. TMAC-03101Alias proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (2C751) is a Rabbit antibody targeting PCNA. Anti-PCNA Antibody (2C751) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-PCNA Antibody (2C751) is a Rabbit antibody targeting PCNA. Anti-PCNA Antibody (2C751) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | proliferating cell nuclear antigen, ATLD2 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PCNA on different lysates using anti-PCNA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293, Lane 3: A431. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin. 8. Flow cytometric analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:100-500; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 29 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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