Anti-PCMT1 Antibody (9L379) is a Mouse antibody targeting PCMT1. Anti-PCMT1 Antibody (9L379) can be used in WB,ICC/IF.

IgG1, Kappa

9L379

Human

1. Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-10071
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate:1: HeLa, 2: 293T
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 27 kDa
Observed MW: 27 kDa

WB: 1:500-1000; ICC/IF: 1:100-500

Monoclonal

PCMT1

Protein-L-isoaspartate(D-aspartate) O-methyltransferase

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).