Anti-PCK1 Antibody (9P530) is a Mouse antibody targeting PCK1. Anti-PCK1 Antibody (9P530) can be used in WB,IHC-P,IHC-Fr,IF.

IgG1, Kappa

9P530

Human,Rat

1. Tissue: Rat liver
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-10068
2. Tissue: Human liver
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-10068
3. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room temperature 2 h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: Human liver, 2: Rat kidney
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 65 kDa
Observed MW: 65 kDa

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

PCK1

phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.